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6×105 cath a cells  (ATCC)
94
ATCC 6×105 cath a cells
6×105 Cath A Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotin tagged cath ka bound streptavidin resin  (Thermo Fisher)
99
Thermo Fisher biotin tagged cath ka bound streptavidin resin
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
Biotin Tagged Cath Ka Bound Streptavidin Resin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin tagged cath ka bound streptavidin resin/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
biotin tagged cath ka bound streptavidin resin - by Bioz Stars, 2026-03
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operator cath volume  (Genovis Inc)
99
Genovis Inc operator cath volume
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
Operator Cath Volume, supplied by Genovis Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/operator cath volume/product/Genovis Inc
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operator cath volume - by Bioz Stars, 2026-03
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leader-cath arterial catheter 4f  (Vygon Company)
90
Vygon Company leader-cath arterial catheter 4f
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
Leader Cath Arterial Catheter 4f, supplied by Vygon Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/leader-cath arterial catheter 4f/product/Vygon Company
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leader-cath arterial catheter 4f - by Bioz Stars, 2026-03
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operator cath  (Genovis Inc)
99
Genovis Inc operator cath
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
Operator Cath, supplied by Genovis Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/operator cath/product/Genovis Inc
Average 99 stars, based on 1 article reviews
operator cath - by Bioz Stars, 2026-03
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cath b  (Sino Biological)
93
Sino Biological cath b
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
Cath B, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cath b/product/Sino Biological
Average 93 stars, based on 1 article reviews
cath b - by Bioz Stars, 2026-03
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cath a  (ATCC)
94
ATCC cath a
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
Cath A, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cath a/product/ATCC
Average 94 stars, based on 1 article reviews
cath a - by Bioz Stars, 2026-03
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Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured with biotin-tagged Cath-Ka-bound Streptavidin resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Cathelicidin-Ka, the first frog-derived TLR2 and TLR4 agonist, induces macrophage activation and promotes inflammation

doi: 10.1007/s00018-025-06068-y

Figure Lengend Snippet: Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured with biotin-tagged Cath-Ka-bound Streptavidin resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant

Article Snippet: In brief, RAW264.7 cells (1 × 10 7 cells) at logarithmic growth period were lysed with Pierce IP lysis buffer from Thermo Fisher Scientific (Waltham, MA, USA) and incubated with biotin-tagged Cath-Ka-bound Streptavidin resin overnight at 4°C according to the manufacturer’s instructions (EZ-Link Desthiobiotinylation and Pull-Down Kit, Thermo Fisher Scientific Company).

Techniques: Flow Cytometry, Binding Assay, Labeling, Cell Culture, Enzyme-linked Immunosorbent Assay, Recombinant, Negative Control, Control